Date of Assessment:	Conducted By:
Building:	Room Number:	Department:
Principal Investigator (PI):

Instructions: This form must be completed by the PI, Lab Supervisor, or their designee to conduct a laboratory hazard assessment specific to activities in their laboratories. The laboratory hazard assessment identifies hazards to employees and specifies personal protective equipment (PPE) to protect employees during work activities. The person conducting the assessment must verify that it is complete and that all applicable training has been conducted.

Item #	Work Activity Using…	Are the stated activities performed in the lab?	Potential Hazard	Applicable Engineering Controls	Applicable Administrative Controls	Applicable PPE
Yes	No
CHEMICAL HAZARDS
Small volumes (10mL – 4L) of corrosive liquids.	Eye or skin damage.	Chemical fume hood	Completion of annual lab safety training Review Chemical Hygiene Plan (CHP)	Chemical splash goggles Light chemical-resistant gloves (Rubber Latex). Lab coat
Large volumes (>4L) of corrosive liquids, small to large volumes (< 10 mL- >4L) of acutely toxic corrosives, or work which creates a splash hazard.	Poisoning; increased potential for eye and skin damage.	Chemical fume hood	Completion of annual lab safety training Review Chemical Hygiene Plan (CHP)	Chemical splash goggles, face-shield Heavy chemical-resistant gloves (Butyl or Viton). Lab coat and chemical resistant apron
Small volumes (10mL – 4L) of organic solvents or flammable organic compounds.	Skin or eye damage, potential poisoning through skin contact.	Chemical fume hood	Completion of annual lab safety training Review Chemical Hygiene Plan (CHP)	Chemical splash goggles Light chemical-resistant gloves (Rubber Latex). Lab coat
Large volumes (>4L) of organic solvents, small to large volumes of very dangerous solvents, or work which creates a splash hazard.	Major skin or eye damage, potential poisoning through skin contact. Fire.	Chemical fume hood	Completion of annual lab safety training Review Chemical Hygiene Plan (CHP)	Chemical splash goggles Heavy chemical-resistant gloves (Butyl or Viton). Flame-resistant lab coat (e.g. Nomex)
Toxic or hazardous chemicals (solid, liquid, or gas).	Skin or eye damage, potential poisoning through skin contact.	Chemical fume hood	Completion of annual lab safety training Review Chemical Hygiene Plan (CHP)	Chemical splash goggles Light chemical-resistant gloves (Rubber Latex). Lab coat, Respiratory protection may be needed
Acutely toxic or hazardous chemicals (solid, liquid, or gas).	Increased potential for eye or skin damage increased potential poisoning through skin contact.	Chemical fume hood Inert atmosphere (glove box)	Completion of annual lab safety training Review Chemical Hygiene Plan (CHP) Designated work areas Prior approvals where applicable	Chemical splash goggles Heavy chemical resistant gloves (Butyl or Viton). Lab coat Respiratory protection may be needed
An apparatus with contents under pressure or vacuum.	Eye or skin damage.	Chemical fume hood	Completion of annual lab safety training	Safety glasses or goggles, face shield for high risk activities Chemical-resistant gloves (Latex, Nitrile, or Butyl) Lab coat, chemical-resistant apron for high risk activities
Air or water reactive chemicals.	Severe skin and eye damage. Fire.	Inert atmosphere (glove box) Storage in areas free of water and/or moisture	Completion of annual lab safety training Review Chemical Hygiene Plan (CHP)	Work in inert atmosphere, when possible Chemical splash goggles Chemical-resistant gloves Lab coat, flame-resistant lab coat for high risk activities (e.g. Nomex) Chemical-resistant apron for high risk activities
Potentially explosive chemicals.	Splash, detonation, flying debris, skin and eye damage. Fire.	Chemical fume hood	Completion of annual lab safety training Review Chemical Hygiene Plan (CHP)	Chemical splash goggles, face shield, and blast shield Heavy gloves Flame-resistant lab coat (e.g. Nomex)
Temperatures below 0˚C.	Burns, splashes, frostbite.	NA	Completion of annual lab safety training Review Chemical Hygiene Plan (CHP)	Chemical splash goggles Thermal insulated gloves when needed Lab coat
Temperatures above 90˚C.	Burns, splashes, fires.	NA	Completion of annual lab safety training Review Chemical Hygiene Plan (CHP)	Chemical splash goggles Thermal insulated gloves when needed. Lab coat Fire retardant PPE
Minor chemical spill cleanup.	Skin or eye damage, respiratory damage.	NA	Completion of annual lab safety training CEPAR “Just In Time Guide”	Chemical splash goggles Chemical resistant gloves (Nitrile). Lab coat Chemical-resistant apron and boot/shoe covers for high risk activities Respirator as needed Consider keeping Silver Shield gloves in the lab spill kit
BIOLOGICAL HAZARDS
Human blood, body fluids, tissues, or bloodborne pathogens (BBP).	Exposure to infectious material.	Biosafety cabinet	Completion of annual lab safety training Completion of annual BBP training Review of BBP-Exposure Control Plan Hepatitis B Vaccine	Face shield or facemask with goggles (if splash risk) Latex or nitrile gloves Lab coat or gown
Preserved animal and/or human specimens.	Exposure to infectious material or preservatives.	Biosafety cabinet	Completion of annual lab safety training Completion of annual BBP training Review of BBP-Exposure Control Plan Hepatitis B Vaccine	Chemical splash goggles Protective gloves such as light latex or nitrile for unpreserved specimens (select protective glove for preserved specimens according to preservative used) Lab coat or gown
Radioactive human blood, body fluids, or bloodborne pathogens (BBP).	Cell damage, potential spread of radioactive contaminants, or potential BBP exposure.	Biosafety cabinet	Completion of annual lab safety training Completion of annual BBP training Completion of Radiation Safety training Review of BBP-Exposure Control Plan Hepatitis B Vaccine	Safety glasses (goggles for splash or aerosol hazard) Light latex or nitrile gloves Lab coat or gown.
Manipulation of cell lines, viruses, bacteria, recombinant / synthetic nucleic acid molecules (rDNA) or other organisms in a Biosafety Level (BSL) 1 facility.	Eye or skin irritation.	NA	Completion of annual lab safety training Completion of annual BBP training (if human materials involved) Completion of Biosafety training Review of Biosafety Manual	Safety glasses (goggles for protection from splash) or other eye hazard Light latex or nitrile gloves for broken skin or skin rash Lab coat or gown
Manipulation of cell lines, viruses, bacteria, recombinant / synthetic nucleic acid molecules (rDNA) or other organisms in a BSL-2 facility.	Exposure to infectious material, particularly through broken skin or mucous membranes.	Biosafety cabinet Autoclave	Completion of annual lab safety training Completion of annual BBP training (if human materials involved) Completion of Biosafety training Review of Biosafety Manual	Chemical splash goggles for protection from splash or other eye hazard Light latex or nitrile gloves Lab coat or gown
Manipulation of cell lines, viruses, bacteria, recombinant / synthetic nucleic acid molecules (rDNA) or other organisms in a BSL-2 facility with BSL-3 practices (BSL-2+).	Exposure to infectious materials with high risk of exposure by contact or mucous membranes.	Biosafety cabinet Autoclave	Completion of annual lab safety training Completion of annual BBP training (if human materials involved) Completion of Biosafety training Review of Biosafety Manual	Chemical splash goggles for protection from splash or other eye hazard Light latex or nitrile gloves (double) Lab coat or disposable gown (preferred) Surgical mask
Manipulation of cell lines, viruses, bacteria, recombinant / synthetic nucleic acid molecules (rDNA) or other organisms in a BSL-3 facility.	Exposure to infectious materials with high risk of exposure, particularly through the inhalation route.	Biosafety Cabinet Autoclave	Completion of annual lab safety training Completion of annual BBP training (if human materials involved) Completion of Biosafety training Review of Biosafety Manual	Chemical splash goggles for protection from splash or other eye hazard Light latex or nitrile gloves (double) Full disposable gown or Tyvek suite (preferred) Respirator Shoe cover or dedicated shoe
Work with live animals housed in an Animal Biosafety Level (ABSL) 1 facility.	Animal bites, allergies.	Biosafety cabinet	Completion of annual lab safety training Completion of applicable animal safety training Completion of CITI training Review DAR specific procedures	Safety glasses (goggles for protection from splash) Latex, nitrile or vinyl gloves for broken skin or skin rash Lab coat or gown Consider need for wire mesh glove
Work with live animals housed in an ABSL-2 facility.	Animal bites, exposure to infectious material, allergies.	Biosafety cabinet (not applicable to larger animals such as non-human primates).	Completion of annual lab safety training Completion of applicable animal safety training Completion of CITI training Review DAR specific procedures	Chemical splash goggles for protection from splash or other eye hazard Face shield Latex, nitrile and/or bite resistant gloves Solid front gown Head cover Shoe covers Surgical mask Consider need for wire mesh glove
Work with live animals housed in an ABSL-3 facility.	Animal bites, exposure to infectious materials with high risk of exposure, particularly through the inhalation route, allergies.	Biosafety cabinet (not applicable to larger animals such as non-human primates).	Completion of annual lab safety training Completion of applicable animal safety training Completion of CITI training Review DAR specific procedures	Chemical splash goggles for protection from splash or other eye hazard Face shield 2 pairs of latex, nitrile, or vinyl gloves Bite resistant gloves Solid front lab gown or Tyvek suit Head cover Shoe covers Possibly respiratory protection Consider need for wire mesh glove
RADIOLOGICAL HAZARDS
Solid radioactive materials or waste.	Cell damage, potential spread of radioactive materials.	Chemical fume hood	Completion of annual lab safety training Completion of radiation safety training Review of Radiation Safety Manual	Safety glasses Latex or nitrile gloves Lab coat
Radioactive materials in hazardous chemicals (corrosives, flammables, liquids, powders, etc.).	Cell damage or spread of contamination plus hazards for the specific chemical.	Chemical fume hood	Completion of annual lab safety training Completion of radiation safety training Review of Radiation Safety Manual	Chemical splash goggles Light chemical-resistant gloves Lab coat Note: Select glove for the applicable chemical hazards.
Ultraviolet radiation	Conjunctivitis, corneal damage, skin redness.	UV shield	Completion of annual lab safety training Review of Chemical Hygiene Plan (CHP)	UV face shield and goggles Lab coat
Infrared emitting equipment (e.g. glass blowing)	Cataracts, burns to cornea.	NA	Completion of annual lab safety training	Appropriate shaded goggles Lab coat
LASER HAZARDS
Open Beam
Performing alignment, troubleshooting or maintenance that requires working with an open beam and/or defeating the interlock(s) on any Class 3 or Class 4 laser system.	Eye damage.	NA	Completion of annual lab safety training Completion of laser safety training Review of Laser Safety Manual	Appropriately shaded goggles/glasses with appropriate optical density at the appropriate electromagnetic wavelength; based on individual beam parameters
Viewing a Class 3R laser beam with magnifying optics (including eyeglasses).	Eye damage.	NA	Completion of annual lab safety training Completion of laser safety training Review of Laser Safety Manual	Appropriately shaded goggles/glasses with optical density based on individual beam parameters
A Class 3B laser open beam system with the potential for producing direct or specular reflections.	Eye damage, skin damage.	Beam enclosures Beam attenuators Beam blocks, or Laser rated curtains	Completion of annual lab safety training Completion of laser safety training Review of Laser Safety Manual	Appropriately shaded goggles/glasses with optical density based on individual beam parameters Appropriate skin protection
A Class 4 laser open beam system with the potential for producing direct, specular, or diffuse reflections.	Eye damage, skin damage.	Beam enclosures Beam enclosures Beam attenuators Beam blocks, or Laser rated curtains	Completion of annual lab safety training Completion of laser safety training Review of Laser Safety Manual	Appropriately shaded goggles/glasses with optical density based on individual beam parameters Appropriate skin protection
Non-Beam
Handling dye laser materials, such as powdered dyes, chemicals, and solvents.	Cancer, explosion, fire.	Chemical fume hood	Completion of annual lab safety training Completion of laser safety training Review of Chemical Hygiene Plan (CHP)	Gloves Chemical splash goggles Flame-resistant lab coat or coveralls
Maintaining and repairing power sources for large Class 3B and Class 4 laser systems.	Electrocution, explosion, fire.	NA	Completion of annual lab safety training Completion of laser safety training Completion of electrical safety training Maintenance and repairs should be completed by vendor	Electrical isolation mat Flame-resistant lab coat or coveralls
PHYSICAL HAZARDS
Cryogenic liquids.	Major skin, tissue, or eye damage.	NA	Completion of annual lab safety training Review of Chemical Hygiene Plan (CHP)	Goggles for large volumes Impermeable insulated gloves (Cryogenic - Waterproof). Lab coat
Removing freezer vials from liquid nitrogen.	Vials may explode upon rapid warming. Cuts to face/neck and frostbite to hands.	NA	Completion of annual lab safety training Review of Chemical Hygiene Plan (CHP)	Face shield Impermeable insulated gloves (Cryogenic). Lab coat
Very cold equipment or dry ice.	Frostbite, hypothermia.	NA	Completion of annual lab safety training Review of Chemical Hygiene Plan (CHP)	Safety glasses Insulated gloves (Cryogenic) (possibly warm clothing) Lab coat
Hot liquids, equipment, open flames (i.e., autoclave, Bunsen burner, water bath, oil bath).	Burns resulting in skin or eye damage.	NA	Completion of annual lab safety training Review of Chemical Hygiene Plan (CHP)	Safety glasses or goggles for large volumes Insulated gloves (impermeable insulated gloves for liquids, steam) Lab coat
Glassware washing.	Lacerations; burns.	NA	Completion of annual lab safety training	Chemical splash goggles Heavy rubber gloves Lab coat Leather closed toed shoes
Working with loud equipment, noises, sounds, alarms, etc.	Potential ear damage and hearing loss.	NA	Completion of annual lab safety training	Earplugs or ear muffs as determined by EHSO noise monitoring
A centrifuge.	Imbalanced rotor can lead to broken vials, cuts, exposure, and spills.	NA	Completion of annual lab safety training	Chemical splash goggles Latex, vinyl, or nitrile gloves Lab coat
A sonicator.	Ear damage, exposure.	NA	Completion of annual lab safety training Noise monitoring if applicable Audiometric testing if applicable	Safety glasses or goggles Latex, vinyl, or nitrile gloves Lab coat Earplugs or ear muffs as determined by EHSO noise monitoring
Sharps (including broken glass).	Cuts, exposure.	NA	Completion of annual lab safety training Review of BBP-Exposure Control Plan Review of Sharps Guidelines	Safety glasses or goggles Latex, vinyl, or nitrile gloves Lab coat Leather closed toed shoes
Moving compressed gas cylinders.	Crushed foot/ toes, punctured valve causing torpedo.	NA	Should be done only by vendor	Steel toe guards or steel toed boots
NANOMATERIAL HAZARD
Unbound engineered solid nanomaterials.	Inhalation, exposure, dermal exposure.	NA	Completion of annual lab safety training	Goggles Gloves (change frequently) Arm sleeves (gantlets) Lab coat Respiratory protection may be needed
Unbound engineered liquid nanomaterials, where aspiration possible.	Inhalation, exposure, dermal exposure.	NA	Completion of annual lab safety training	Goggles Gloves Lab coat Respiratory protection may be needed

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