Salmonella Typhimurium

Family Enterobacteriaceae; S. typhimurium is a gram negative rod; motile, aerobic and anaerobic. This bacteria is one of the non-typhoid strains of salmonella common in the US.

LB broth/agar (37°C); Aerobic.

Humans, patients with acute illness and chronic carriers, flies possibly as a vector.

Skin exposure; mucous membrane splash; ingestion.

Acute infectious disease with sudden onset of abdominal pain, diarrhea, nausea and vomiting.

Unknown.

Usually 6 to 72 hours for gastroenteritis and 5-21 days for enteric fever.

None available.

None available for non-typhoid Salmonella species.

Antibiotic treatment is specific to symptomatology.

Monitor for symptoms; bacteriological examination of blood, excreta.

Report all incidents. Contact the Biosafety Officer to discuss the need for a CDC and/or USDA permit.

Laboratory infections have been reported with Salmonella spp.

Contaminated food, blood, abscesses, lesion exudates, CSF, feces, and urine.

Salmonella Information

Biosafety in Microbiological and Biomedical Laboratories

Salmonella Infections

Salmonella Guidance Documents & Regulatory Information

All work with Salmonella-infected samples or propagation of the bacteria must be conducted inside a Biological Safety Cabinet (BSC) Class II. Use face shield, surgical mask and eye protection for open bench work.

Hand washing procedures must be carefully followed.

Animal work should be conducted in the BSC. 

Risk assessment will be needed for procedures generating aerosols.

Notify others working in the lab. Allow aerosols to settle. Don appropriate PPE. Cover area of the spill with paper towels and apply an EPA-registered disinfectant, working from the perimeter towards the center. Allow 30 minutes of contact time before disposal and cleanup of spill materials.

For assistance, contact Emory’s Biosafety Officer (404-727-8863), or the EHSO Spill Team (404-727-2888).

Exposure should be irrigated vigorously.

Immediately go to the sink and thoroughly wash the wound with soap and water for 15 minutes. Decontaminate any exposed skin surfaces with an antiseptic scrub solution.

Remove garments that may have become soiled or contaminated and place them in a double red plastic bag.

Immediately report incident to supervisor, complete an employee incident report in PeopleSoft.

7am-4pm (OIM): EUH (404-686-7941) EUHM (404-686-7106) WW (404-728-6431)

After Hours: OIM NP On Call (404-686-5500) PIC# 50464

Needle Stick (OIM): EUH (404-686-8587) EUHM (404-686-2352)

Yerkes: Maureen Thompson Office (404-727-8012) Cell (404-275-0963)

1% sodium hypochlorite, 70% ethanol.

Sensitive to heat, UV light, Blue light-emitting diodes (LEDs).

Survival ranges from hours to months.

At minimum, personnel are required to don gloves, closed toed shoes, lab coat, and appropriate face and eye protection prior to working with Salmonella typhimurium. Additional PPE may be required depending on lab specific SOPs.

All procedures that may produce aerosols, or involve high concentrations or large volumes should be conducted after consultation with the Biosafety Officer. The use of needles, syringes, and other sharp objects should be strictly limited. Additional precautions should be considered with work involving animals or large scale activities.